Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 61)

4. To apply sample, fill the syringe and insert it into the open female luer of the 3-way
stopcock. Turn the 3 way stopcock lever up (toward the top inlet fitting), and inject the
sample into the loop.
5. Return the 3-way stopcock lever to the middle position, facing toward the syringe. The
desalting loop is now filled with sample and ready for the desalting method to begin.
Fig. 6.29. Sample loop installed in the cartridge 1 position.
Note: Determine the appropriate combination of desalting loop and desalting cartridge for
the protein sample volume to be applied. For a 2 ml sample, choose the 2 ml desalting loop
and the 10 ml desalting cartridge. For a 10 ml sample, choose the 10 ml desalting loop and
the 50 ml desalting cartridge. Application of a sample volume smaller than the desalting
loop volume is not recommended, since it will result in increased dilution of the sample and
reduced desalting resolution, desalting resolution. If desired, small volume samples can be
diluted to 2 or 10 ml for buffer exchange applications.
6.7 Starting the Run
The Start Run screen (Figure 6.30) displays a summary of the sample information. A
reminder prompts you to connect the instrument to a computer running Profinia software if
you would like to view the chromatogram in real time (see section 12 for more information
on Profinia software). The Profinia system does not require software to operate the instrument;
current data points appear on the run screen.
The lower toolbar contains buttons for viewing the methods steps and for optional manual
priming of any extra tubing attached to the sample, buffer, diluent, or waste lines to
accommodate larger containers in these instrument ports (see section 11.2 for details).
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