Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 218)

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Method Type:
Method Name:
eXact Cartridge Size:
Desalting Cartridge Size:
Sample 1 Name & Volume:
Sample 2 Name & Volume:
Method Step Step Description
Position/
Reagent
Buffer
Conc.,
1-5X
Frac
#
(S1/S2)
Flow
Rate
ml/min CV
Peak
Detect
Delay
Max
Peak
Detect
Vol
Flow
Rate
ml/min CV
Peak
Detect
Delay
PRIMING STEPS Not Editable x w/w x x x x x x x
EQUILIBRATING C2
Equilibrate Di-C2 Equilibrate DS Cartridge
with Di water Di Water x w/w x x x
Equilibrate C2 Equilibrate DS Cartridge
with desalting buffer
B4/Desalting
buffer w/w x x x
EQUILIBRATING C1
Equilibrate C1 Equilibrate with
Equilibration/Wash-1 buffer
B1/equilibration
Wash-1buffer w/w x x x
LOADING SAMPLE
Load Sample to C1 Load sample to eXact
cartridge C1 x 1A/2A x x x x x
WASHING
Wash-1 C1/C2 Wash eXact cartridge with
Equilibration/Wash-1 buffer
B1/equilibration
Wash-1buffer 1B/2B x x x
Wash-2 C1/C2
(for 2-Wash methods)
Wash eXact cartridge with
Wash-2 buffer B2/Wash-2
buffer 1C/2C x x x
INCUBATION-ELUTE to DS
Prime Elution buffer Not Editable B3/Elution buffer x w/w x x x x x x x
Pre-EluteC1 Fill eXact Cartridge with
Elution buffer
B3/Elution buffer
w/w
Incubation Enter 0 to 999 hrs. x __Hrs x x x __Hrs x
Elute-1 C1 Start elution until affinity
peak is detected
B3/Elution buffer
w/w x x
Elute-2 C1 Divert affinity peak To DS B3/Elution buffer w/w x x x
ELUTION DS
Elute-1 C2 Start elution until protein
peak is detected B4/Desalt buffer w/w x x
Elute-2 C2 Collect protein peak B4/Desalt buffer 1D/2D x x x
Elute-3 C2 Remove salts from DS B4/Desalt buffer w/w x x x
CARTRIDGE CLEAN
Clean-1 C2 Clean Desalt Cartridge B5/Clean-1 solution w/w x x x
Clean-2 C2 Clean Desalt Cartridge
B6/Regeneration
buffer w/w x x x
Clean-1 C1 Clean eXact Cartridge
B1/equilibration
Wash-1buffer w/w x x x
Clean-2 C1 Clean eXact Cartridge
B6/Regeneration
buffer w/w x x x
Clean-3 C1 Clean eXact Cartridge
B1/equilibration
Wash-1buffer w/w x x x
STORING
Store C1 Store eXact Cartridge B7/Storage solution w/w x x x
Store C2 Store Desalt Cartridge B7/Storage solution w/w x x x
SAMPLE 2 SETTING
Sample 1&2
Settings
Peak Parameter
Settings
Peak Pa
Setti
SAMPLE 1 SETTINGS
eXact Affinity + Desalting Program Method Planning Worksheet