Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 9)

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• Built-in cleaning and storage steps — automated system cleaning ensures continued
peak instrument performance
• Purification kits — affinity and desalting cartridges, buffers, and solutions optimized
for Bio-Rad methods
• Optional cooling accessory — samples and fractions of purified samples stay cold
without keeping the instrument in a cold room or using a cold box
• Optional Profinia software — real-time data and customized reports can be displayed, used for
basic protein comparisons and analysis, and incorporated into lab notebooks and
presentations
1.3 System Components and Optional Accessories
The Profinia protein purification system components (see Figure 1.1) are:
Profinia instrument — shipment box #1
Profinia accessory kit — shipment box #2; includes:
• Profinia buffer bottle starter pack — 4 x 125 ml and 4 x 250 ml empty bottles
• Profinia buffer lids — pack of eight lids
• Profinia lids for 15 ml sample conical tubes — pack of two lids
• Profinia lids for 50 ml sample conical tubes — pack of two lids
• Profinia waste/diluent (water) bottle pack — 2 x 2 L bottles
• Profinia cleaning tray — placed in the fraction collection area to collect liquid by
products from line cleaning and system wash procedures
• Inline filter — 12 replacement filters
Profinia starter kit — shipment box #3; choice of native IMAC or GST starter kit, includes
1 ml affinity cartridge, 10 ml desalting cartridge, vial of Profinia control lysate, and a complete
buffer set for native IMAC or GST purification
Fig. 1.1. Profinia purification system components.
IMAC or
GST
starter kit
Cleaning
tray
Profinia
instrument
Buffer bottles
Waste and
diluent
(water)
bottles
Buffer and
sample lids
Inline filter