Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 192)

Date
Maintenance Procedure Performed Comments
4. Replace the UV lamp, if necessary
(section 13.2.3).
5. Check UV flow cell assembly (section
13.2.4).
After replacing any system parts or
components, run the system ethanol wash,
then the procedure to flush all inlet and
outlet lines (section 13.1.4).
Recheck the pump flow rate and note the
results.
Note that the pressure reads zero with the
system stopped; re-zero the sensor if
necessary. Install a calibrated gauge and
20 psi backpressure regulator at the pressure
sensor outlet. Compare the readings of the
gauge and the sensor, recalibrate the sensor
if not within ±4 psi. Replace the pressure
sensor if calibration is not successful.
Replace the back panel and reconnect all
tubing and cables.
If desired, run a starter kit separation to
validate system operation.
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