Bio-Rad Profinia™ Protein Purification Instrument User Manual
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Fig. 6.40. Run Completed screen.
6.10.1 Exporting Data to a Computer
The run data file can be transferred to a portable memory device as an .ofi file, which can be
imported into Profinia software (optional) for viewing data in tabular form or in a chromatogram.
If Profinia software has not been purchased, the data file can be viewed in any compatible
spreadsheet software (for example, Microsoft Excel). To export the run data file:
1. Insert a USB portable memory device into the USB port directly below the touch screen.
The Export Data button is initially displayed with an “X” across it, indicating that the
button is not yet functional. As soon as the system recognizes the portable memory
device, the “X” disappears and the Export Data button is available. The time required
for recognition depends on the portable device used and can take up to 15 sec. (See
troubleshooting in Appendix C if the memory device is not recognized).
2. Touch the Export Data button in the lower right corner of the End of Run screen. A
progress bar displays indicating that the transfer is in progress; typical transfer times
are approximately 60 sec. The actual transfer time depends on the amount of data in
the run data file and the portable memory device.
Upon completion of data export, you will automatically return to the Run Completed screen.
6.10.2 Performing Final Run Procedures
The toolbar at the bottom of the Run Completed screen displays final steps to shut down the
system or prepare for a new run. Choose one of the following options:
•
Re-Run Method — immediately begin a new sample run with the same method
•
Run New Method — immediately begin another purification run using a different
method type, or new buffers for the run that was just performed
•
Short-Term Wash — if the system will not be used again on the same day, the buffer
lines and valves should be rinsed with water
•
Long-Term Wash — if the system will not be used in the next 2 days, it should be
rinsed with water and ethanol or other sanitizer to maintain optimum mechanical
performance and prevent bacterial growth
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