Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 6)

9.1.2 Real-Time Data Transfer to Profinia Software ...............................93
9.2 System Utilities ........................................................................................93
9.2.1 Calibrating Touch Screen .............................................................94
9.2.2 Checking Pump Flow Rate ...........................................................94
9.2.3 Calibrating UV Monitors ................................................................95
9.2.4 Calibrating Conductivity Monitor....................................................96
9.2.5 Adjusting Peak Detection Sensitivity .............................................97
9.2.6 Calibrating pH Probe ....................................................................97
9.2.7 Calibrating Air Detection System...................................................98
9.2.8 Calibrating Pressure .....................................................................98
9.3 Diagnostic/Maintenance Functions ..........................................................98
9.3.1 Displaying Firmware Versions.......................................................99
9.3.2 Setting and Operating Instrument Manually ..................................99
9.3.3 Setting Time and Date ................................................................104
9.3.4 Cleaning Sample Lines ...............................................................105
9.3.5 Cleaning Fraction Lines ..............................................................106
9.3.6 Cleaning Buffer Lines..................................................................107
9.3.7 Washing System With Ethanol....................................................108
9.3.8 Cleaning Inlet and Outlet Lines ...................................................109
9.3.9 Cleaning Pump Check Valves.....................................................110
9.3.10 Washing System With NaOH ......................................................111
9.3.11 Cleaning Inline Check Valve .......................................................112
9.3.12 Affinity Cartridge Cleaning in Place .............................................112
9.3.13 Desalting Cartridge Cleaning in Place.........................................113
9.3.14 Selecting End of Sample/Reagent Detection (Air Sensors) .........114
9.3.15 Selecting Method Temperature...................................................115
9.3.16 Select Display Contrast...............................................................116
Section 10 Profinia Purification Kits...........................................................117
10.1 Introduction............................................................................................117
10.1.1 Background ................................................................................117
10.1.2 Product Information ....................................................................117
10.1.3 Equipment and Materials Required .............................................118
10.1.4 Storage Conditions .....................................................................118
10.2 Native IMAC Purification and Buffer Kits ................................................118
10.3 Denaturing IMAC Purification and Buffer Kits .........................................120
10.4 GST Purification and Buffer Kits.............................................................122
10.5 Desalting Buffer and Separation Kits......................................................123
Section 11 Connecting External Components...........................................125
11.1 pH Monitor .............................................................................................125
11.2 Larger Buffer, Sample, and Fraction Containers ....................................128
11.2.1 External Buffer Containers ..........................................................128
11.2.2 External Sample Containers .......................................................129
11.2.3 External Fraction Collection Containers ......................................130
11.3 Profinia Cooling Accessory ....................................................................130
11.4 Connecting non-Bio-Rad Cartridges ......................................................132
Section 12 Software.....................................................................................133
12.1 Operating Requirements........................................................................133
12.2 Software Structure .................................................................................133
12.3 Transferring Data to Profinia Software ...................................................134
12.3.1 Real-Time Data Transfer ............................................................134
12.3.2 Portable Memory Device Data Transfer ......................................135
iii