Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 41)

Note: Two samples can be run for all methods except the desalting-only method. When
two samples are selected for affinity-only methods, the number of cartridges option
becomes available. You have the option to use a single cartridge for both samples or a
separate cartridge for each sample. When two samples are selected for affinity with desalting
methods, both samples are purified using the same cartridge set. Desalting-only methods
are run with one sample that is applied to the cartridge via a sample loop placed in the
cartridge 1 position. See section 6.6.5 for details on the installation and use of the desalting
loop.
Note: When purifying two samples through the same cartridge, the method cleans and
equilibrates the cartridge prior to loading the second sample.
3. Touch the arrow button to the right of the Cartridge Options list to select the appropriate
cartridge size for your method. Select to install “1” or “2” cartridges when this option
becomes available.
Note: The “1” or “2” cartridge selection option is only available with two-sample, affinity-only
methods.
When finished setting the method type and options for the run, touch Next to proceed to the
Select Sample parameters screen, or Select Incubation Time screen.
Note: Sample loading flow rate and wash time options are not required with the desalting-
only method; you will proceed directly to the Enter Run & Sample Information screen
(see Section 6.4).
6.3 Selecting Sample Options
6.3.1 Selecting Sample Flow Rate and Wash Time
Each IMAC, GST, and Protein A and G Bio-Rad method is available with an option to
select from two sample loading flow rates and two cartridge wash times. The Select
Sample Flow Rate & Wash Time screen (Figure 6.6) is used to select the sample loading
flow rate and wash time for optimum protein yields and purity (for example, with a high
molecular weight protein, slower flow rates may improve protein binding efficiency and an
extended wash time may improve protein purity). Flow rates and wash options can be
selected separately for each sample (up to two) used in the run.
Fig. 6.6. Select Sample Flow Rate & Wash Time screen with options displayed for a two-sample method.
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