Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 200)

Section 4
Ordering Information
Catalog # Description
732-0111 Luer to M6 Adaptor Fittings Kit
732-0112 Luer to 10-32 Adaptor Fitting Kit
732-0113 Luer to BioLogic System Fittings Kit
750-0603
PTFE
FEP Tubing, 1/8”, for pre-pump connections, 15’
731-8211 Silicone Tubing , 1.6 mm ID, 0.8 mm wall
620-0401 Profinia Cooling Accessory
620-0404 Profinia Inline Filter Assembly Replacement
620-0228 Profinia Desalting Kit
620-0224 Profinia Desalting Buffer Kit , 10 applications
732-4600 Bio-Scale Mini Affi-Prep Protein A Cartridges , 5 x 1 ml
732-4602 Bio-Scale Mini Affi-Prep Protein A Cartridges , 1 x 5 ml
732-5302 Bio-Scale Mini Profinity Desalting Cartridges , 1 x 10 ml
732-5312 Bio-Scale Mini Profinity Desalting Cartridges , 1 x 50 ml
620-0216 5x Profinia Desalting Buffer , 200 ml
620-0217 2x Profinia Cleaning Solution 1 , 125 ml
620-0218 4x Profinia Cleaning Solution 2 , 125 ml
620-0219 2x Profinia Storage Solution , 200 ml
Section 5
Protocol for Protein A Elution/Regeneration Buffer
Preparation
500 mM Sodium Citrate, pH 3:
For 250 ml, add the following to 200 ml of high quality water:
19.75 g Anhydrous citric acid
6.53 g Sodium citrate dihydrate
Stir solution until thoroughly dissolved. Adjust buffer volume to 0.25 L, filter sterilize
(0.2 micron filter), and transfer to sterilized bottles compatible with the Profinia buffer ports.
1750 mM Glycine, pH 2.5:
For 250 ml, add the following to 160 ml of high quality water:
32.84 g Glycine
Stir solution until thoroughly dissolved then adjust to pH 2.5 by titration with 6 N HCl
(~29.5 ml). Use appropriate precautions when working with HCl (wear gloves and goggles
and work in a well ventilated area or hood). Adjust the buffer volume to 0.25 L, filter sterilize
(0.2 micron filter), and transfer to sterilized bottles compatible with the Profinia buffer ports.
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