Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 30)

4.2 Profinia ™ System Quick Guide
23
Note: An “X” indicates that no reagent
or container is required in that position.
Place bottle
caps here
during the run
Buffer lid
on bottle
Numbers on
bottle labels
match
numbers on
instrument
Buffer bottles are properly placed in all
positions and lid is closed in
preparation for instrument operation.
Native IMAC buffer set is shown.
To start a preprogrammed purification run, touch Bio-Rad Methods
button on the home screen.
Step 1: Select Method Type and Sample Information
1. Select the method system on the Select Method System screen.
2. Select the method type on the Select Method Type & Options
screen
3. Select sample loading and washing options on the Select Sample
Flow Rate & Wash Time screen.
4. Enter information on the Enter Run & Sample Information screen.
Step 2: Install Reagents, Sample Tubes, and Fraction Tubes
Install bottles and tubes as indicated on the Install Reagents,
Sample & Fraction Tubes screen.
2A. Installation of Reagents/Buffer Bottles
1. Get the reagents from the Profinia purification kit designed for the
selected method.
2. Add any dry materials required to the reagents and filter; see
purification kit manual for details.
3. Place a green buffer lid on each buffer bottle.
4. Open the top cover of the Profinia instrument to access sipper
tubes and buffer placement tray.
5. Lift the sipper tube away from the instrument.
6. With the number code on the bottle label facing out, insert the
bottle onto the sipper tube for each position.
7. Fill a 250 ml bottle with 20% ethanol and place it in position 8.
8. Close the Profinia top cover during operation.