Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 85)

When all edits are completed, touch the Save button in the Enter Run & Sample
Information screen to store your method in system memory. As your method is written to
memory, a popup screen appears with a “Working — Please Wait” message.
Note: You can also run the edited method without saving it to memory.
If all run and sample information has been satisfactorily updated, touch the Next button.
You will proceed to the Install Reagents, Sample & Fraction Tubes screen.
7.5 Installing Reagents, Sample Tubes, and Fraction Tubes
The Install Reagents, Sample & Fraction Tubes screen (Figure 7.8) displays a diagram
showing where buffer, diluent, sample, fraction, and waste containers should be placed in
the Profinia instrument (cartridge installation is described in section 6.6). This diagram is
specific to the method type and options selected for the run. The volumes indicated
for the buffer, diluent, and waste bottles are the minimum volumes required. Volumes
indicated for the fractions are those that will be delivered to the fraction tubes. An “X” in any
position indicates that no reagent or container is required in that position. The empty waste
container displayed on the diagram serves as a reminder to empty the waste container
prior to starting a run.
Note: Adjustments made to methods during programming may result in buffer, diluent, or
waste bottle and sample or fraction tube volume requirements greater than the capacities
of the standard bottles and tubes provided with the instrument. For instructions on modifying
the system to accommodate larger bottles and tubes, see section 11.2.
Fig. 7.8. Install Reagents, Sample & Fraction Tubes screen (left) with arrows demonstrating the correlation
between the diagram and placement of reagents in the Profinia instrument (right).
The installation screen diagram displays:
• Buffer bottles — eight buffer bottle positions indicated by labels B1 through B8 on the
touch screen (top of the screen) and 1 through 8 on the instrument (upper tray). The
touch-screen diagram also displays the minimum buffer volumes required for the selected
run; an “X” indicates no reagent is required at that location. Three dashes (---) followed
by “ml volume” indicates the required volume exceeds 999 ml
Note: If the reagent volumes exceed the container capacity, a larger container can be con-
nected to the instrument externally. When reagents are connected externally make sure to
prime the lines manually immediately prior to starting the run. The manual priming function
is available in the Start Run screen.
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