Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 125)

Table 10.1. Profinia purification and buffer kits.
Kit Catalog # Solutions Powdered Reagents Cartridges
Purification Desalting Storage
Buffers
Buffer Solutions Urea Glutathione Affinity Desalting
Profinia Native IMAC Kits
Native IMAC
purification kit
620-0225
✔ ✔ ✔
✔ ✔
Native IMAC
buffer kit
620-0221
✔ ✔ ✔
Profinia Denaturing IMAC Kits
Denaturing IMAC
purification kit
620-0227
✔ ✔ ✔
Denaturing IMAC
buffer kit
620-0222
✔ ✔✔
Profinia GST Kits
GST purification 620-0226
✔ ✔ ✔ ✔ ✔ ✔
kit
GST buffer kit 620-0223
✔ ✔ ✔ ✔
Profinia Desalting Kits
Desalting 620-0228
✔ ✔
✔
kit
Desalting 620-0224
✔ ✔
buffer kit
10.1.3 Equipment and Materials Required
Profinia purification and buffer kits are specifically designed to be used with the Profinia
protein purification instrument. All of the buffers and solutions come formulated as
concentrates that are made with chromatography-grade solutions, and have been prefiltered
to remove particulates. The buffers that require the addition of urea or glutathione also
require filtration through a 0.2 µm filter prior to use with the system. Accessories to the
Profinia instrument include Profinia software, cooling accessory units, and desalting sample
loops. Consumables that can be purchased separately include Profinia bacterial lysis and
extraction reagents, His and GST detection antibodies, and a dual-tagged lyophilized
control lysate containing GST and histidine residues. Complete catalog number and ordering
information can be found in Appendix B.
10.1.4 Storage Conditions
All unopened Profinia IMAC purification and buffer kits can be stored between 4 and 22°C.
The GST kits can be stored at 22°C for up to two weeks. For long-term storage, the GST
kits should be stored at 4°C. The labels on the outside of the kit box provide exact expiration
dates, and each kit is guaranteed for ~1 year when stored at the proper temperature. Once
opened, the kits and reagents should be stored at 4°C.
10.2 Native IMAC Purification and Buffer Kits
The buffers provided in the native IMAC kit are formulated from potassium salts and
buffers, and contain increasing concentrations of imidazole for optimum lysing, binding,
washing, and elution of histidine (His)-tagged proteins.
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