Bio-Rad Profinia™ Protein Purification Instrument User Manual

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Section 5
Home Screen Overview

The LCD touch screen on the front of the instrument is used to control Profinia

system

functions (Figure 5.1). You may use your finger or a stylus to operate the screen. The
home screen displays date and time information and the UI firmware version number. The
upper right corner of each set-up screen displays various status indicators (see section 3.4).

The toolbar at the bottom of the home screen displays five navigation buttons for the
available user options: Bio-Rad Methods, Program Methods, Saved Methods, Data/Utilities,
and i (information).

Note: The toolbar remains at the bottom of each system screen; however, button names
and their respective functions will change to reflect the requirements of each screen.

Fig. 5.1. Profinia system home screen.

Home screen toolbar options are:

Bio-Rad Methods — access preprogrammed method templates for affinity chromatography,
with or without desalting steps. These methods include native and denaturing IMAC, GST
purifications, Protein A and G purifications, Profinity eXact purifications, and desalting-only
methods

Program Methods — customize Bio-Rad method templates by editing the parameters of
method steps. A program method can be saved to permanent memory

Saved Methods — access saved program methods. Saved program methods (up to 35 at
a time) can be retrieved and viewed by method type or as a single, alphabetical list

Data/Utilities — retrieve run data files, calibrate the system, and run maintenance and
diagnostic functions:

Data — system memory stores up to three run data files; run data files are in .ofi
format. Stored run data files are available for transfer via a USB portable memory
device (USB flash drive). These run data files can then be imported into the Profinia
software program (optional system accessory). Stored run data files that have been
transferred to a USB portable memory device are indicated with an asterisk

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