Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 113)

9.3.5 Cleaning Fraction Lines
This procedure is used to clean the eight fraction lines; buffer position 8 holds the 20%
ethanol solution and is used as a final system purge:
1. Touch the Data/Utilities button on the system home screen.
2. Touch the Diag/Maint Functions button.
3. On the Diag/Maint Functions screen, touch the up and down arrows to scroll through
the list of functions and highlight “Clean Fraction Lines”, then touch the Select button.
4. Follow the directions displayed on the screen (Figure 9.9).
5. Touch the Start button. The system purges the fraction lines with water followed by a
system purge with ethanol from buffer position 8. A progress bar displays while cleaning
is in process.
Note: This procedure always cleans all eight fraction lines.
When cleaning is complete, the system returns to the Diag/Maint Functions screen.
Fig. 9.9. Prepare system to clean fraction lines screen.
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