Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 158)

Fig. 13.8. Pulling the pump head away from the drive assembly.
7. Using your fingers, remove the pump wash fittings from the top of the pump head.
8. Remove both pistons and piston carriers from the pump head by pulling them out with
your fingers.
9. Use the piston seal tool to remove both main seals located at the bottom of the recess
where the piston carriers were removed from by inserting the tip of the tool in the seal,
and gently prying sideways until the seal pops loose from the recess (Figure 13.9).
Fig. 13.9 Removing the main seals.
Note: Once a seal is removed, it cannot be reused.
10. Rinse the pump head in deionized water.
11. It is best to soak the new seals in 50% alcohol (methanol, isopropanol, or ethanol).
Sonicate in alcohol if possible. This removes any air that might be trapped in the seal.
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