Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 217)

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Method Type:
Method Name:
eXact Cartridge Size:
# Cartridges:
Sample 1 Name & Volume:
Sample 2 Name & Volume:
Method Step Step Description
Position/
Reagent
Buffer
Conc.,
1-5X
Frac
#
(S1/S2)
Flow
Rate
ml/min CV
Peak
Detect
Delay
Max
Peak
Detect
Vol
Flow
Rate
ml/min CV
Peak
Detect
Delay
Max
Peak
Detect
Vol
PRIMING STEPS Not editable x w/w x x x x x x x x
EQUILIBRATING C1/C2
Equilibrate C1/C2 Equilibrate with
equilibration/wash-1 buffer
B1/equilibration
wash-1 buffer w/w x x x x
LOADING SAMPLE
Load sample to C1 Load sample to eXact
cartridge C1 x 1A/2A x x x x x
Load sample to C2 Load sample to eXact
cartridge C2 x 2A x x x
WASHING
Wash-1 C1/C2 Wash eXact cartridge with
equilibration/wash-1 buffer
B1/equilibration
wash-1 buffer 1B/2B x x x x
Wash-2 C1/C2
(for 2-wash methods)
Wash eXact cartridge with
wash-2 buffer B2/wash-2
buffer 1C/2C x x
INCUBATION-ELUTION
Prime elution buffer Not Editable B3/elution buffer x w/w x x x x x x x x
Pre-elute C1-C2 Fill eXact cartridge with
elution buffer
B3/elution buffer
w/w
Incubation Enter 0 to 999 hrs. x __Hrs x x x __Hrs x x
Elute-1 C1/C2 Start elution until affinity
peak is detected
B3/elution buffer
w/w x x
Elute-2 C1C2 Collect affinity peak B3/elution buffer 1D/2D x x x x
CARTRIDGE CLEAN
Clean-1 C1/C2 Clean eXact cartridge
B1/equilibration
wash-1buffer w/w x x x x
Clean-2 C1/C2 Clean eXact cartridge
B6/regeneration
buffer w/w x x x x
Clean-3 C1/C2 Clean eXact cartridge
B1/equilibration
wash-1 buffer w/w x x x x
STORING
Store C1/C2 Store eXact cartridge B7/storage solution w/w x x x x
Peak Parameter
Settings
Peak Parameter
Settings
SAMPLE 1 SETTINGS SAMPLE 2 SETTINGS
Sample 1&2
Settings
eXact Affinity only Program Method Planning Worksheet