Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 64)

2. Open Profinia software on your computer by double-clicking on the desktop Profinia
software icon. Look for the “Waiting for data” message in the lower left corner of the
software screen. This indicates the instrument is communicating with the software and
a run can be started (Figure 6.33).
Fig. 6.33. “Waiting for data” message on the bottom of the Profinia software screen.
At this point, the run can be started and the chromatogram appears on the computer
screen.
Important: The real-time USB data connection cannot be established once the Start button
has been touched and a run has been started. However, the run data file can be exported
to a USB portable memory device and transferred to Profinia software after the completion
of the run.
6.7.3 Clearing System Memory to Start the Run
To start your purification run:
Touch the Start button. The Preparing Data Memory Storage screen displays a progress
bar indicating temporary memory data clearance. The system can store up to three run
data files in memory. An “M” status indicator displayed in the upper right corner of the
screen indicates none of the run data files stored in system memory have been
transferred. If system memory is full (indicated by “F” in the status position), the oldest
transferred or stored data file will be overwritten with the new file when a purification run is
started.
Fig. 6.34. Touch Start on the Start Run screen to begin a purification run.
57