Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 68)

Fig. 6.36. Touch the Manual Peak Divert button at the step in the run indicated by the arrow to optimize
concentration of the eluted protein.
When the protein peak has eluted (Figure 6.36), you can touch the Manual Peak Divert button
(Figure 6.37) to automatically advance to the next step without waiting for the completion of
that step.
Fig. 6.37. Run in Progress screen with Manual Peak Divert button displayed.
6.8.3 Collecting the Purified Sample
The purified sample is collected in fraction tube position 1D (sample 1) or 2D (sample 2).
When the purified protein is collected, the Run in Progress screen displays the actual
elution times and tubes 1D and 2D can be removed. After the target protein has eluted, the
Profinia system automatically continues to run the cartridge cleaning and storage procedures
and purify the second sample if selected.
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