Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 149)

4. If system power will be left on, go to the Manual Operation screen (Data/Utilities >
Diag/Maint Functions > Manual Operation) and turn the lamp off, using the Toggle
Lamp button.
Note: When the Profinia instrument is switched on, the UV lamp does not start until a
method starts. The UV lamp shuts off automatically one hour after a method is finished. If
you use the Toggle Lamp button to switch the lamp on, it will stay on until the machine is
powered down or until you toggle the lamp off. The one hour shutoff does not function
when the lamp is turned on manually.
13.1.4 System Cleaning Procedures
Cleaning Sample Lines
Use this procedure to clean the lines between the inlet select valve and the sample tubes:
1. Remove all sample tubes from the instrument.
2. Fill conical tubes with 25 ml of deionized water and place them in sample positions 1
and 2.
3. Fill a buffer bottle with 100 ml of 20% ethanol and place it in buffer position 8.
4. Touch the Start button. A progress bar displays, tracking the duration of the procedure.
Cleaning Fraction Lines
Use this procedure to clean the lines between the outlet select valve and the fraction tubes:
1. Remove all fraction tubes from the instrument.
2. Place the cleaning tray in the fraction collection area.
3. Place a bottle with 100 ml of 20% ethanol in buffer position 8.
4. Touch the Start button. A progress bar displays, tracking the duration of the procedure.
Cleaning Buffer Lines
Use this procedure to clean the lines between the buffer containers and the inlet select
valve:
1. Fill seven buffer bottles with 50 ml of deionized water each, and place them in buffer
positions 1 through 7.
2. Fill one buffer bottle with 100 ml of 20% ethanol and place it in buffer position 8.
3. Touch the Start button. A progress bar displays, tracking the duration of the procedure.
System Ethanol Wash
Use this procedure to pump ethanol solution through the system:
1. Remove both cartridges from the system, and connect the inlet and outlet cartridge
fittings together.
2. Fill seven buffer bottles with 50 ml of 20% ethanol each, and place them in buffer
positions 1 through 7.
3. Fill one buffer bottle with 100 ml of 20% ethanol and place it in buffer position 8.
4. Fill two conical tubes with 25 ml of 20% ethanol each, and place them in sample
positions 1 and 2.
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