Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 88)

• Install Cartridges — displays the required cartridges and their respective positions
When cartridge installation is complete, touch the Next button to proceed to the Start Run
screen.
7.6.1 Installing the Sample Loop (Desalting-Only Methods)
Warning: When the desalting-only method is selected, DO NOT fill the sample loop with
sample until the sample loop and cartridge installation is completed. Inject the sample
immediately prior to starting the run.
Sample loading for a desalting-only method is achieved via a sample loop. Installation is
directed by the Install Bio-Scale Mini Cartridges screen (Figure 7.10). The desalting sample
loop accessory connects to the instrument in the cartridge 1 position and the desalting
cartridge in the cartridge 2 position (Figure 7.12).
Fig. 7.10. Install Bio-Scale Mini Cartridges screen showing sample loop installation in the cartridge 1
position.
To connect and fill the desalting loop:
1. Insert the male luer fitting from cartridge holder 1 into the top female luer fitting of the
3-way stopcock. Ensure that the second female luer fitting of the 3-way stopcock is
facing out so the syringe may be easily inserted.
2. Connect the male luer of the end of the desalting loop to the female luer fitting of cartridge
holder 1.
3. Install the desalting cartridge in the cartridge 2 position. Touch the Next button to proceed
to the Start Run screen (Figure 7.10).
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