Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 87)

Note: Reagent volumes that exceed system container sizes can be used in external
containers connected to the instrument via extra tubing. Make sure to manually prime these
lines prior to starting the run. The Start Run screen has a Manually Prime button in the
lower toolbar (see section 7.8.2).
7.5.1 Preparing and Installing Reagents and Samples for the Purification Run
To install program method reagents:
1. Fill each buffer bottle with the minimum volume as indicated on the screen.
2. Place a buffer lid on each buffer bottle and install each buffer bottle as indicated on the
screen.
Note: Buffer lids help keep contaminants out of the buffer bottles and guide sipper tubes
into the bottles.
3. Position the buffer bottles with the graduated marks facing out, making it easier to
check buffer volume during the method (see section 6.5.1 for proper buffer bottle
orientation).
4. Add sample to the sample tube(s).
5. Place sample lids on the sample tubes and position the sample(s) in the instrument.
Important: Each run primes the sample line with about 1 ml of sample. To ensure that the
full amount is loaded onto the cartridge, fill the sample tubes with an additional 1 ml than
the entered volume. If the exact entered volume is added to the sample tube, the end of sample
detection, if activated, applies the complete sample to the system. In this case, the total
amount of sample added to the cartridge will be approximately 0.5 ml less than the entered
volume. The Run Completed screen displays the actual amount of sample that was loaded
onto the cartridge.
6. Make sure the diluent bottle is filled with sufficient deionized water and the waste
container is empty or has sufficient space to hold the generated waste.
7. Install the fraction tubes as indicated on the screen diagram. If the volume required
exceeds the instrument capacity, connect external collection containers using extra
tubing (see section 11.2).
When all the reagents, sample(s), and fraction tubes have been installed according to the
installation diagram, touch the Next button to proceed to the Prepare for Cartridge
Installation screen.
7.6 Installing Cartridges
Prior to installing the required cartridges, the instrument cartridge lines must be primed to
prevent introduction of air bubbles into the cartridge. The cartridge installation routine
consists of four consecutive screens (see section 6.5 for complete cartridge installation
instructions):
• Select Cartridge Status — includes the option to bypass the installation
procedure when the cartridges are already installed (for example, from a previous run)
and proceed directly to the Start Run screen
• Setup Cartridge Positions for Priming — directs connection of the cartridge system
fittings at positions 1 and 2
• Priming in Progress — displays the priming time progress bar
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