Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 120)

3. On the Diag/Maint Functions screen, touch the up and down arrows to scroll through
the list of functions and highlight “Affinity Cartridge Cleaning in Place”, then touch the
Select button.
4. Select the size of the cartridge to be cleaned (1 ml or 5 ml).
5. Follow instructions displayed on the screen (Figure 9.16).
6. After the cartridge and reagents have been installed press the Start button.
7. At the end of the cleaning method the user has the option to end or repeat the method.
(Repeating the method will subject the cartridge to another cleaning cycle).
The cleaning in place method steps are listed in Table 9.1. Note that 20% aqueous ethanol
needs to be supplied at buffer position 8. This is for system cleaning only and is not part of
the cleaning in place method. The flow rate for 1 ml and 5 ml cartridges is 1 ml/min and 5
ml/min, respectively.
Table 9.1. Steps of the Affinity Cleaning in Place Method
Buffer Buffer Buffer Column Flow Rate (ml/min)
Port Conc. Volumes 1 ml Cartridge 5 ml Cartridge
Equilibration B5 1x 2 1 5
Cleaning B6 1x 10 1 5
Equilibration B5 1x 5 1 5
Storage B7 1x 5 1 5
20% Ethanol B8 1x – 1 5
Fig. 9.16. Affinity Cartridge Cleaning in Place screen.
9.3.13 Desalting Cartridge Cleaning in Place
A cleaning in place method for 10 ml and 50 ml Bio-Gel P6 desalting cartridges is available
through the Profinia Data/Utilities Menu for storage or cleaning with user defined reagents.
This method uses 1x buffer concentrates at predefined flow rates of 4 and 20 ml/min for 10 ml
and 50 ml cartridges respectively. For Profinia coldroom setting the flow rates are reduced
to 2 and 10 ml/min to prevent overpressure (Table 9.2 and Figure 9.17).
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