Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 46)

Fig. 6.9. Edit Lot Number Information screen.
3. Touch the arrow buttons to the right of the lot number information text box to scroll
through the list of kit information (buffers, solutions, and cartridges) relevant to the run.
Highlight the item for which you want to add lot number information.
4. Touch the Edit button to retrieve the alphanumeric keypad.
5. Enter desired information into the keypad text line.
6. Touch OK to save input data and return to the Edit Lot Number Information screen, or
Cancel to return to the Edit Lot Number Information screen without saving changes.
7. Repeat this process for each item that requires lot number information.
8. Touch Clear All to delete all information added, or OK to accept changes and return to
the Enter Run & Sample Information screen.
When all run and sample information has been satisfactorily updated, touch the Next button.
You will proceed to the Install Reagents, Sample & Fraction Tubes screen.
6.5 Installing Reagents, Sample Tubes, and Fraction Tubes
The Install Reagents, Sample & Fraction Tube screen (Figure 6.10) displays a diagram
showing where buffer, diluent, sample, and waste containers should be placed in the
Profinia instrument (cartridge installation is described in Section 6.6). This diagram is
specific to the method type and options selected for the run. The volumes indicated
for the buffer and water bottles are the minimum volumes required. Volumes indicated for
the fractions are those that will be delivered to the fraction tubes. An “X” in any position
indicates that no reagent or container is required in that position.
Note: If the reagent volumes exceed the container capacity, a larger container can be
connected to the instrument externally. When reagents are connected externally make sure
to prime the lines manually immediately prior to starting the run. The manual priming function
is available in the Start Run screen.
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