Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 165)

Fig. 13.19. Filter carrier and UV lamp carrier.
11. Place the lamp and filter carriers on the UV module body and secure with the screws
using the 9/64" Allen wrench provided with the new lamp.
12. Plug the lamp’s cord into its receptacle.
13. Replace the access panel and waste bottle, reconnect the power cord, and turn the unit
around to face you. The system is now ready to operate.
13.2.4 UV Monitor Flow Cells
The Profinia system automatically cleans the flow path after every run, so the flow cells do
not generally require service. If the flow cells should become dirty or clogged, the most
common symptom is a noisy UV baseline. To check the flow cells, check the UV signal and
reference voltages, as described in section 13.2.3. With the flow cell flushed with clean
water, the sample voltage should be at least 65% of the reference voltage.
Removing and Replacing the UV Monitor Flow Cells
The Profinia UV flow cells are located inside the instrument cabinet, in the UV monitor
assembly (Figure 13.13). To inspect or replace the flow cell:
1. Use the procedure in section 13.2 to remove the instrument access panel.
2. Locate the UV monitor assembly.
3. Remove the two tubing fittings from the top of the flow cells; mark them so they can be
reconnected to the same flow cell (Figure 13.20).
158
UV light
window
Securing
Screw
Holes for
Securing
Screw
Filter-Shiny
Side Faces
UV Lamp