Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 175)

Appendix A
Profinia
™ System Specifications
Operating limits
Flow rate range 0.2–20 ml/min
Maximum back pressure 45 psi (3.4 bar)
Detection
UV detection 280 nm
Conductivity monitor range 0–500 mS/cm
pH monitor pH range 1–14
Sample and Fraction 15 or 50 ml conical tubes and ability to use
Collection containers external reservoirs connected by tubing
Buffer selection Rotary selector valve for up to 8 buffers
Solvent compatibility All commonly used chromatographic solvents*
Operating temperature 4–30°C; cold room compatible
Data output USB mass storage device
Direct USB cable to PC
Dimensions (W x D x H) 58 x 33 x 67 cm (22.7 x 13 x 26.4 in)
Weight 28 kg (62 lb)
Optional accessories**
Cooling accessory
Optional Profinia PC software
Desalting sample loops
pH monitor
*Chemical Compatibility (Chemical/Buffer Concentration)
8 M Urea 1 M Hydrochloric acid
6 M Guanidine hydrochloride 3 M Ammonium sulfate or saturated solution
30% Ethanol 30 mM ß-mercaptoethanol
100% Methanol 10% SDS in PBS
5 M Sodium chloride 5% Triton in PBS
2 M Sodium citrate Aqueous buffers, pH 2–12
30% Glycerol
2 M Sodium hydroxide
3 M Sodium acetate
** Sold separately
168