Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 23)

• Conc. — buffer position in the instrument and buffer concentration (the instrument
dilutes concentrated buffers)
• ml/min — flow rate displayed in ml/min
• CV — column volume based on the cartridge size (for example, for a 1 ml IMAC
cartridge, 2 CV = 2 ml)
• Min — duration of each step in minutes
Fig. 3.2. View Method Steps screen during a 10 ml desalting method run.
3.4 System Status Indicator Definitions
Status indicator symbols are displayed in the top right corner of the screen. The possible
indicators are C, M, A, P, and F:
• C — operating temperature setting. Methods including desalting steps have different
optimum flow rates at room temperature than when under cold room conditions. “C”
indicates that the flow rate limits are set for cold room conditions, and will display only for
methods including desalting when the method temperature is set for 4°C. The method
temperature setting option is located in the Data/Utilities menu (section 9.3.13).
• M — data memory status. The Profinia system stores up to three run data files in memory.
This symbol indicates that the three stored files have not yet been exported to a computer
via a portable memory device. If none of the files have been transferred, the next run
data file will overwrite the oldest stored file
• A — end of reagent detection status. This indicates that some or all end of reagent
detectors have been disabled. Select Data/Utilities to access the end of reagent detection.
• P — program mode status. This indicates that the program method was selected.
• F — method memory status. This indicates that method memory is full.
Note: It is possible that the system may display more than one of these symbols as
appropriate during the course of a Bio-Rad or program method run.
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