Bio-Rad Profinia™ Protein Purification Instrument User Manual

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Table 4. Buffer and Chemical Compatibilities for the Profinity eXact Tag System

1.4 Recommended Storage Conditions

Purchased buffers obtained from Profinia purification kits should be stored between
4–22°C. Once used with the instrument, all reagents should be stored at 4°C for up to 3
months. After insertion into the instrument, the solutions are no longer sterile. Prior to use, it
is important to check solutions for particulates and clarity before reuse. If there is any
indication of particulates, contamination, or microbial growth, the solutions should be
discarded. Bio-Rad Profinity eXact and P6 Desalting Bio-Scale Mini cartridges should be
stored at 4°C in the appropriate storage solutions listed in Table 2.

Section 2
eXact Method Guidelines

2.1 Sample Preparation Information

Bio-Rad Profinity eXact cartridges are convenient, ready-to-use devices for the capture,
cleavage, and purification of proteins expressed from the eXact system expression vector.
The Profinity eXact resin utilizes an immobilized, engineered protease that both recognizes

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