Bio-Rad ChromLab™ Software - Manual (Page 100)

4 | Performing a Manual Run
98 | NGC Chromatography Systems and ChromLab Software
3. To purge an individual line of air bubbles and fill it with buffer:
a. Select a port to open.
b. (Optional) Change the default flow rate. The default flow rate is set to
20
ml/min.
c. Click Start.
d. Repeat steps 3a–c for any other port to purge.
4. To purge all lines of air bubbles and fill them with buffer:
a. Click All.
b. (Optional) Change the default flow rate. The default flow rate is set to
20
ml/min.
c. Click Start.
5. Monitor the pump pressure and outlet flow to ensure that the fluid is flowing
properly.
The system pumps and the buffer blending valve start operating at the
specified flow rate, exchanging the solution in the inlet lines and expelling any
trapped bubbles, cycling sequentially through the ports Q1 to Q4. After the
specified duration, the pumps stop momentarily while the blending valve
switches to port Q3 in order to flush the lines with water before closing the
valve. No change is allowed during this process for the specified duration.
Note: You can stop the cycle before it completes by clicking Stop. Before
stopping the purge cycle, ensure that all buffer lines are filled with the
appropriate buffers before starting a run.
Gradient via
Blending Valve
(High Flow) Buffer blending valve ports Q1 and Q4 are used as inlets for
buffers A and B. Gradients are formed by the buffer blending
valve rather than by the pumps. This enables each pump to
run at their full flow rate capacity which doubles the total
flow rate, for example to 20 ml/min for F10 pumps.
Mode Description