Bio-Rad ChromLab™ Software - Manual (Page 288)

B | Multicolumn Purifications
286 | NGC Chromatography Systems and ChromLab Software
Multicolumn Purification Method Templates
ChromLab™ software includes several multicolumn purification templates. The
templates comprise preprogrammed methods for binding and eluting samples from
multiple columns in an automated sequence. They also offer multidimensional
chromatography strategies whereby the target fraction eluted from one column is
loaded onto another for a second dimension of purification.
You can customize the preprogrammed templates by defining, among others, the
following variables:
 Column type and size for your sample
 Method base unit
 Sample volume
 Step duration and length
Multicolumn Sequential Purification Templates
Sequential binding and elution is used when multiple samples must be purified on
multiple columns. The samples are injected sequentially either by using a sample
pump with sample inlet valve or through sample loops. Each sample is loaded onto
a column and washed to remove contaminants that can cause sample degradation.
The columns are then eluted using either step or linear gradient protocols in a
sequence. The fractions are collected with the BioFrac™ fraction collector or an
outlet valve. The sequential purification templates primarily utilize affinity techniques
but can be modified to include other techniques.
Table 7 on page 287 defines the multicolumn sequential purification templates.