Bio-Rad ChromLab™ Software - Manual (Page 258)

7 | Evaluating Results
256 | NGC Chromatography Systems and ChromLab Software
Displaying Column Performance Results — the
Column Performance Table
The results of the column performance analysis are detailed in the Column
Performance table. The table displays the following fields for the column
performance peak:
 N — displays the calculated number of theoretical plates for the column.
 N/L (cm
-1
) — displays the calculated number of theoretical plates per
meter for the column.
 HETP (cm) — displays the calculated height equivalent to a theoretical
plate for the column.
 rHETP — displays the calculated reduced plate height for the column.
 Bed height (cm) — displays the value for medium bed height as specified
in the Settings pane.
 Particle diameter (

m) — displays the value for average particle diameter
as specified in the Settings pane.
 Run start time
 Column type — displays the type of column. The column type is set in the
Method Editor tab in the Method Settings phase.
 Start — the beginning of the peak, displayed in the units of the currently
chosen x-axis.
 End — the end of the peak, displayed in the units of the currently chosen
x-axis.
 Retention time — displays the elution time at maximum peak height.
 Retention volume — displays the elution volume at maximum peak
height.
 Height (mAU) — displays the peak height at the retention volume/time,
measured from the UV trace to the calculated baseline.