Control flow tab – Bio-Rad ChromLab™ Software User Manual
Page 74
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| System Control
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| NGC Chromatography Systems and ChromLab Software
7.
If your system includes both the BioFrac fraction collector and an outlet valve,
select both checkboxes. In the Include Outlet valve – 50
l section, specify the
tubing length between the UV outlet port and the outlet valve as well as the
tubing length between the outlet valve and the fraction collector drophead.
8.
Select or clear the next four checkboxes, depending on whether these modules
are present on your system.
Note:
If a pH detector module is present on your system and you select
the checkbox to include its cell volume, determine whether the detector is
set to Inline or Bypass mode and select the appropriate radio button.
9.
If one or more of the options is not applicable, or if you change the length of the
tubing, enter the appropriate values in the Additional volume box.
The system calculates the delay volume based on your specifications and
displays the results in
l at the bottom of the dialog box.
10. Click OK.
Control Flow Tab
Use the option Control Flow to avoid overpressure to prevent the system from
exceeding the maximum pressure limit. Control Flow reduces the flow rate by half
whenever the system pressure gets within 80% (the default) of the maximum
system pressure limit. Control Flow lowers the flow rate multiple times if necessary.
However, it will not reduce the flow rate below the minimum user-specified flow rate.
For methods, Control Flow resets the flow rate at the start of each phase and then
readjusts it if necessary. The pumps are immediately stopped if the pressure
exceeds the maximum pressure limit at any time.
The option Control Flow to avoid overpressure is available in the System Pump and
Sample Pump dialog boxes in manual mode as well as in the Method Editor
window. You can choose either of these settings before performing a run or running
a method to control the flow rate within the pressure limit range using settings in the
Control Flow tab of the System Settings dialog box.