Bio-Rad ChromLab™ Software - Manual (Page 106)

4 | Performing a Manual Run
104 | NGC Chromatography Systems and ChromLab Software
To set up the experiment in the fluidic scheme
1. Double-click the Fraction Collector module and choose the following settings in
the dialog box that appears.
a. In the Rack Type dropdown list, select the installed rack type.
b. Under Collection Scheme, select the fraction collection Start Rack and
Tube, number of Tubes to Collect, and Fraction Size.
c. Press Collect.
2. (Optional) Double-click the pH module, if available, and set the valve position:
 Select Bypass to bypass the pH electrode.
 Select pH to direct flow through the pH flow cell and monitor the pH during
the run.
3. Double-click the Single- or Multi-Wavelength UV Detector plus Conductivity
Monitor module, choose the following settings, and then close the dialog box.
a. Select the wavelengths for monitoring the experiment: 280 nm for proteins
containing tryptophans and tyrosines; 255 nm for nucleic acids; and, for
multi-wavelength detection only, 215 nm for the amide bond of peptides.
b. Turn the lamp On if it is off.
c. Click Zero Baseline to zero the UV lamp.