Bio-Rad ChromLab™ Software - Manual (Page 183)

Scouting on Parameters within a Method
User Guide | 181
The Scouting Wizard
The scouting wizard guides you through three pages: Choose a Scouting
Parameter, Select Method Steps to Scout, and Generate Scout Sequence. This
section explains how to use each page in detail.
Page 1: Choose a Scouting Parameter
On this page you select the parameter on which to scout. You can select only one
parameter per method to scout.
 Flow Rate — optimize the pump flow rate for adsorption and elution
steps.
 %B — vary the buffer composition in isocratic gradient steps or the
initial or final buffer composition in linear gradient steps.
 Duration — vary the length of the elution step in volume.
 pH — find the optimal pH for the method in buffer blending mode (requires
a buffer blending valve).