Bio-Rad ChromLab™ Software - Manual (Page 95)

User Guide | 93
4 Performing a Manual Run
The NGC™ chromatography system instrument operates in two modes: manual
mode and automated, programmed method mode. In manual mode you have full
control of each NGC pump, valve, detector, and fraction collector. This mode is
used primarily for nonautomated processes such as priming and cleaning or
purging the system, but it can also be used to load samples, optimize
chromatography parameters, and run simple experiments.
Method mode enables you to execute preprogrammed steps automatically. See
Chapter 5, Method Editor , and Chapter 6, Creating a Method , for information about
working in method mode.
When the NGC system is turned on, the instrument touch screen displays the Home
window in manual mode. By default the system remains in manual mode unless
calibration is in progress or a method is running.
You can enter the required instrument settings by double-clicking the individual
module dialog boxes in the fluidic scheme. This chapter describes these settings.
See also Chapter 3, Preparing the Instrument, in the NGC Chromatography
Systems and ChromLab Software Instrument Guide for more detailed information
on tasks described in this chapter.
Manual tasks can be performed in the System Control window using the computer
connected to the NGC instrument or on the touch screen. Runs performed manually
can be saved to the ChromLab™ database located on the computer.
Note: The NGC system can be operated in manual mode without a connected
computer.