Table – Bio-Rad ChromLab™ Software User Manual
Page 289
Multicolumn Purification Method Templates
User Guide
| 287
Table 7.
Multicolumn sequential templates
Template Name
Template Description
Affinity (1 ml) — Linear
Gradient
This method is used to purify many samples quickly. Using a
sample inlet valve and the sample pump, the method loads
sample onto separate affinity columns and washes unbound
material off in quick succession. This process can be repeated
for up to five columns. This method is designed to reduce the
target protein’s exposure to proteases. The target proteins are
then eluted from each column sequentially using a linear
gradient.
Affinity (1 ml) — Step (Manual
Load)
This method is used to purify many samples quickly. Using a
loop valve (a column switching valve plumbed to the injection
valve in the loop position with multiple sample loops), the
method loads sample onto separate affinity columns and
washes unbound off material in quick succession. This
process can be repeated for up to five columns. This method
is designed to reduce the target protein’s exposure to
proteases. The target proteins are then eluted from each
column sequentially using a step gradient.
Affinity (1 ml) — Step Gradient
This method is used to purify many samples quickly. Using a
sample inlet valve and the sample pump, the method loads
sample onto separate affinity columns and washes unbound
material off in quick succession. This process can be repeated
for up to five columns. This method is designed to reduce the
target protein’s exposure to proteases. The target proteins are
then eluted from each column sequentially using a step
gradient.
Affinity (5 ml) — Linear
Gradient
This method is used to purify many samples quickly. Using a
sample inlet valve and the sample pump, the method loads
sample onto separate affinity columns and washes unbound
material off in quick succession. This process can be repeated
for up to five columns. This method is designed to reduce the
target protein’s exposure to proteases. The target proteins are
then eluted from each column sequentially using a linear
gradient.