Bio-Rad ChromLab™ Software - Manual (Page 122)

5 | Method Editor
120 | NGC Chromatography Systems and ChromLab Software
Phase Library Pane
The Phase Library pane contains both standard and custom phases you can use to
create methods. Each phase consists of a series of programmed steps that
represent a process in chromatography. You can add or remove steps from a phase
to customize it for a specific application. You can save modified phases as custom
phases in the Custom tab where they will be available to all ChromLab software
users. Standard phases are further described in Standard Phases on page 138 .
Table 4. Standard Phases
Phase Explanation
Method Settings Define the parameters used throughout the
method.
Equilibration Equilibrates the column before or after
elution.
Sample Application Applies sample to the column. Defines
mode of application, either direct or through
sample loop, sample volume, flow rate, and
buffers used for sample loading.
Column Wash Washes out unbound proteins (flow
through) after sample application. When
used after an elution, the conditions defined
would remove proteins bound strongly to
the media under conditions used for
elution.
Elution Elutes the sample from the column.
System CIP (Clean in Place)/
Storage Rinses the system with a cleaning solution.
This phase pulls from a single cleaning or
storage solution.
Note: System CIP must be run as the
only type of phase in the method. You
can add several System CIP phases to
the method if multiple cleaning
solutions are required.