Bio-Rad ChromLab™ Software - Manual (Page 99)

Preparing the System
User Guide | 97
To purge the buffer blending valve
1. In the fluidic scheme, double-click the System Pump module to open its dialog
box.
2. Select Priming in the Mode dropdown list.
 To purge an individual line of air bubbles, proceed to step 3 on page 98.
 To purge all lines of air bubbles sequentially, proceed to step 4 on page 98.
Tip: The available buffer blending valve modes are defined as follows:
Mode Description
Buffer Blending Blends stock acid, base, water, and salt solutions to form
isocratic or linear buffer gradients at a user-specified buffer
concentration and pH using the Buffer Blending module.
Priming Opens the buffer blending inlet ports for priming and turns
the pumps on to flush the inlet line and pumps with the
buffer at all four ports.
Gradient via Inlet
Valves Buffers are selected at the inlet valve. Gradients are made
by pumps A and B using the selected buffers.