Column performance — manual adjustment – Bio-Rad ChromLab™ Software User Manual

Page 257

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Column Performance Analysis

User Guide

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Column Performance — Manual Adjustment

In the Manual Adjustment tab of the Column Settings pane you can manually adjust

the start and end points of the peak within the column performance phase range.

Adjustments to the start and end points appear in the relevant column of the

Column Performance table in the bottom pane. You can also adjust the start and

end points on the Column Performance table. These changes appear at the

appropriate points on the chromatogram.

To change the peak start or end points on the chromatogram

Drag the peak’s start and/or end marker to the adjusted time value.

To change the peak start or end points on the Column Performance table

Type the adjusted time value in the relevant column.

To undo the adjustment

Click Undo in the Manual Adjustment tab.

To redo the adjustment

Click Redo in the Manual Adjustment tab.

Displaying Column Performance Results — the
Chromatogram

The chromatogram displays the column performance analysis peak as hatched and

shaded. The peak’s start, end, and apex are indicated by small vertical lines at the

relevant x points.

The retention time or column volume appears near the line of the peak apex.

Start Peak appears as a continuous vertical line located at the relevant x point.

End Peak appears as a dashed vertical line located at the relevant x point.

Pausing the pointer on a peak start, end, area, or apex displays a tooltip that shows

details of the item.

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