Bio-Rad ChromLab™ Software User Manual
Page 295
Performing Multicolumn Purification Chromatography
User Guide
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Multicolumn Tandem 2-D Affinity Templates
Tasks
1.
Insert a second column switching valve (referred to as the loop
valve) near the sample inject valve on the NGC instrument and map
as C2
2.
Short length of tubing from port F on the sample inject valve to the
left inlet port on the loop valve (C2)
3.
Short length of tubing from port E on the sample inject valve to the
right inlet port on the loop valve (C2)
4.
One 5 ml loop tubing for each affinity column to the top and bottom
ports on the loop valve (C2)
For example, if you have two affinity columns
Attach loop tubing to Column Top port 1 and Column Bottom
port 1
Attach loop tubing to Column Top port 2 and Column Bottom
port 2
5.
Tubing from port 2 on the outlet valve to the syringe port on the
sample inject valve
6.
Affinity columns at ports 1–4 on C1
7.
Desalting or SEC column at port 5 on C1
Multicolumn Tandem Affinity Templates
Tasks
1.
Insert a second column switching valve (C2) into the NGC
instrument
2.
Short length of tubing from outlet port on C1 to the inlet port on C2
3.
Short length of tubing from outlet port on C2 to the UV detector
4.
Affinity columns at ports 1–5 on C1
5.
Desalting column at port 5 on C2