Bio-Rad ChromLab™ Software - Manual (Page 135)

Method Settings Parameters
User Guide | 133
BioFrac Settings — by default, fraction collection begins in the first tube of the
first rack. The fraction size is the default for the rack type.
BioFrac Collection Pattern — by default, Serpentine is selected. For
microplates, fractions can also be collected by rows or columns.
Outlet Valve Settings — by default, fraction collection begins from
Outlet valve 1 (O1) Port 2. O1 Port 1 connects to the BioFrac™ fraction
collector if it is present or directs flow to waste if the fraction collector is absent.
The default fraction size is 50 ml.
Flow
Flow Rate — shows the default rate of flow for the method.
Control the flow to avoid overpressure — monitors the pressure and reduces
the flow rate by 50% when the pressure gets within a certain percentage of the
maximum (default is 80%, see Control Flow Tab in Chapter 3 for more
information).
Detect end of buffer with air sensor — stops the system pumps if air is
detected in any of the lines connected to air sensors set to detect end of buffer.
Air sensor settings are determined on the Air Sensors tab in the System
Settings dialog box. (See Air Sensors Tab in Chapter 3 for more information).
Buffer Selection
Note: When the configuration does not include a buffer blending valve, buffer
settings are inactive.
You can choose one of three Buffer Selection modes:
 Manually Prepared — buffers are user prepared and fed directly into the
pumps or selected by means of buffer inlet valves.
 Manually Prepared via Blending Valve — buffers are user prepared and
fed to the pump through buffer blending valve ports Q1 and Q4. Gradients
are formed by the blending valve rather than the pumps. Because of this,
both pumps can run at their full capacity simultaneously, which doubles
the available flow rate range.