Bio-Rad ChromLab™ Software - Manual (Page 231)

Peak Integration
User Guide | 229
Peak Parameters
You can specify initial values for two parameters that participate in the peak
detection process: slope and filter sensitivity.
Slope — slope defines the threshold for peak slope. The bigger the slope, the
steeper the peaks that are filtered out. Slope values range from 1–100.
Sensitivity — sensitivity determines the strength of the filter used for smoothing the
curve before finding peaks. The Low sensitivity setting filters out higher frequency
components in the signal, eliminating small, narrow peaks. The higher the sensitivity
setting, the more higher frequency components get through the filter. This allows
smaller and narrower peaks to be detected.
Peak Filtering
You can specify the following thresholds for valid peaks.
Min Height — this setting specifies the minimum height for a valid peak in mAU
units.
Min Width — this setting specifies the minimum baseline width for a valid peak in
ml units.
N-Largest — this setting specifies area-wise filtering. It picks N largest peaks in
descending order.
Range — these settings define the range within which peaks will be detected. The
range units are determined from the chromatogram x-axis units.
Default Parameters
Reset — restores the baseline parameters, peak detection parameters, and peak
filtering setting to their defaults.
Note: You must click Integrate to recalculate peaks with the default settings.