Bio-Rad ChromLab™ Software - Manual (Page 176)

6 | Creating a Method
174 | NGC Chromatography Systems and ChromLab Software
Collecting Fractions in Run Queues
When you schedule queued methods, collection begins with the tube or port
defined in the first method in the queue.
Collecting Fractions Using the BioFrac Fraction Collector
After the first method is completed, fraction collection skips a tube and continues
with the next available tube (as if the run queue is set to Append mode). For
example, if Run 01 collects fractions in tubes A1–A10, fraction collection for Run 02
skips tube A11 and begins in tube A12. This process of skipping a tube continues
for each run in the queue.
If a subsequent method in the queue is a multiple run in overlay mode, fraction
collection for the first run skips a tube and starts repeating tubes from the first tube
of the first run in that multiple run.
Note: If a currently running queued method is paused and then continued,
fraction collection continues with the next available tube. If a currently running
queued method is stopped, the queue begins again with the next method. In
this case, fraction collection skips a tube and continues from the next available
tube.
Collecting Fractions Using Outlet Valves
After the first method is completed, fraction collection continues with the next
available port (as if the run queue is set to Append mode). For example, if Run 01
collects fractions from ports P2–P10, fraction collection for Run 02 begins from P11.
This process continues for each run in the queue.
When the last port is used, collection starts at the port defined in the current
method.
Note: If a currently running queued method is paused and then continued,
fraction collection continues with the next available port. If a currently running
queued method is stopped, the queue begins again with the next method. In
this case, fraction collection starts from the port defined in this new method.