Bio-Rad ChromLab™ Software - Manual (Page 207)

Customizing the Chromatogram
User Guide | 205
Showing or Hiding a Trace
To show or hide a trace
 In the trace table, select or clear the Show checkbox in the trace row.
Changing the Axes
The chromatogram has two y-axes. The left axis is the primary axis. Its default trace
is UV absorbance. The default trace of the right axis is Conductivity. You can
change the units of either y-axis by clicking its label, which is a toggle, to show the
next trace’s scale and units.
Alternatively, you can change the units of the primary axis by choosing the active
trace in the chromatogram, as described in Selecting the Active Trace on page 204 .
To change x-axis units
 Click the x-axis title to switch between the available options.
For runs that were performed from saved methods, the x-axis unit can be changed
to Time (min), Volume (ml), or Column Volume (CV). For runs that were performed
manually, the x-axis unit can be changed to Time (min) or Volume (ml).
Controlling the Range of the Y-Scale
For each trace, you can change the maximum and minimum y values in the trace
table to set the chromatogram scale. By default, traces that share the same UV
units have the same y-scale range. When you change the y-scale range (Min Y or
Max Y) of a trace, the new range applies to all traces in the same run that have the
same units. In a display in which traces from different runs are overlaid in a single
chromatogram, changing the y-scale range of one trace changes the y-scale range
of all overlaid traces that have the same units. When traces from each run are
stacked in the display, the scale for each of the stacked chromatograms can be
different.
To change the Max Y and Min Y values in the trace table
 Enter maximum and minimum values in the appropriate trace table row,
ensuring that the maximum value always exceeds the minimum value.