Bio-Rad ChromLab™ Software - Manual (Page 141)

Phase Controls and Parameters
User Guide | 139
Loading the Loop with a Sample Pump
When a sample pump is used to load the loop, you can define the flow rate, the
volume to load into the loop, and, subsequently, the volume of sample to be
injected onto the column. You also have the option to interrupt loading the loop if air
is detected. If you select this checkbox and air is detected in any of the lines
connected to air sensors set to detect end of sample, the system stops the sample
pump and the method continues to the next step.
Fluidic Scheme Includes a Sample Inlet Valve
If your fluidic scheme includes at least one sample inlet valve and you choose to
load the loop through the sample pump, you have the option to perform a
pre-injection wash with buffer to flush the sample pump and lines and the loop. If
you select this checkbox, you can choose the port on the sample inlet valve from
which to draw buffer solution. You can define the flow rate and the volume of buffer
for the preinjection wash.
You also have the option to flush the sample pump and related connections after
sample injection. Again, you can choose the port on the sample inlet valve from
which to draw buffer solution and define the flow rate and the volume of buffer for
the postinjection wash.