Bio-Rad ChromLab™ Software - Manual (Page 128)

5 | Method Editor
126 | NGC Chromatography Systems and ChromLab Software
Column Selection
Under Column Selection you select the column and the column parameters for the
method. The column’s volume and pressure parameters are set automatically when
you select a column type from the list of predefined columns.
If you have one or more column switching valves in the fluidic scheme, you can
choose whether to set the parameters for a single column or for multiple columns.
When multiple columns are used in a method, the method’s phases use the
pressure parameters for any columns that are in line for that phase. You can use
method steps to place two or three columns within the same method. In these
cases, a high-pressure event is triggered when the delta pressure for one of the
column switching valves reaches the high-pressure limit as defined in Method
Settings for the columns that are in line.
You can configure the properties of multiple columns, one for each port on each
column switching valve, and add them to the method using the Configure Ports
dialog box. One column is set as default when you select its checkbox. The method
uses the default's value for column volume throughout the method regardless of any
other columns the method uses. In addition, the method uses the default's
maximum pre- and delta-column pressure until other columns are switched in line. If
two or more columns are switched in line, the method uses the maximum
pre-column pressure of the first column.