Bio-Rad ChromLab™ Software - Manual (Page 155)

Phase Controls and Parameters
User Guide | 153
Column Performance Test Parameters
This test combines the three basic phases necessary for a run (equilibration, sample
application, and elution). After equilibrating the column, a non-adsorbing sample,
such as acetone or salt, is injected via the sample loop and eluted under isocratic
conditions. After the run, calculate the column efficiency in terms of height
equivalent to a theoretical plate (HETP) and the peak asymmetry factor (As).