Bio-Rad ChromLab™ Software - Manual (Page 140)

5 | Method Editor
138 | NGC Chromatography Systems and ChromLab Software
Standard Phases
Some standard phases use identical parameters. For example, the column wash
phase and the elution phase display the same phase parameters. An example of
each phase’s parameters is included in its description.
Note: The images in this section display the parameters and settings for the
NGC Discover Pro fluidic scheme. Your parameters and settings might vary
depending on the fluidic scheme and method in use.
Sample Application Parameters
In sample application phase, you define how a sample gets injected onto a column.
Depending on your system’s configuration, you can apply a sample to the column
manually via a sample loop or directly via the sample pump or autosampler (if
connected).
For all configurations, you have the option to continue injecting a sample until a
particular UV reading is reached. You also have the option to prefill the lines with the
specified volume of buffer when the sample loading buffer is different from the
buffer used in the previous phase.
If your fluidic scheme includes a buffer blending valve and you choose to manually
prepare buffer via inlet valves in Method Settings, you have the option to use the
same inlets as defined in Method Settings or to select specific buffers from each
port.