Bio-Rad ChromLab™ Software - Manual (Page 146)

5 | Method Editor
144 | NGC Chromatography Systems and ChromLab Software
Although it is typically used after the sample application or elution phase, this phase
can be used on its own or at any point that column washing is wanted.
Elution Parameters
In this phase, the sample is eluted from the column using a gradient or isocratic flow
at defined salt concentrations (%B) over a defined volume, column volume, or time.
You can edit the parameters in the gradient segment or add more isocratic or
gradient steps to this segment. This phase can use the flow rate defined globally in
the Method Settings phase or a flow rate different from that of the other phases.
Fraction collection is enabled by default during this phase and offers options to
collect all, to collect when a certain threshold of UV or conductivity has been
reached, or within defined collection windows. When collecting fractions by
threshold, you can opt to collect the fractions that fall outside the defined threshold
range using fraction volumes different from the volumes set for the threshold
fractions.
You can choose an elution technique from the following options:
 Isocratic – define the length of elution (CV, cm/hr, time, volume) and buffer
composition (%B).
 Gradient – define a linear gradient (%B; default = 0–100%) and gradient
slope/duration (CV, time, volume). In this case, the pumps stop at the end
of the set duration.