Bio-Rad ChromLab™ Software - Manual (Page 123)

Phase Library Pane
User Guide | 121
Adding a Phase to a Method
To add a phase to a method
 Do one of the following:
 Drag the phase to the appropriate location in the Method Outline pane.
 Double-click the phase to append it after the currently selected phase in
the outline.
Tip: Ensure that the phase is appended after the selected phase, not
at the end of the method outline. If necessary, reposition the phase by
dragging it into place.
Column Preparation/Activation Before column use, removes the storage
solution and equilibrates the column. By
repeating the phase multiple times, several
preparation solutions can be used one after
another.
Column Performance Test Tests the efficiency of a packed column in
terms of height equivalent to a theoretical
plate (HETP) and the peak asymmetry
factor (A
s
).
System Preparation Before a run, removes storage solution and
fills the system and inlets with buffer
solution. This phase pulls from a single
buffer solution each time the phase is used.
Note: System Preparation must be
run as the only type of phase in the
method. You can add several System
Preparation phases to the method if
cycling between multiple solutions is
required.
Create New Phase Adds steps from the Step Library to create
a phase and store it in the custom phase
library.
Table 4. Standard Phases, continued
Phase Explanation