Bio-Rad ChromLab™ Software User Manual
Page 170
6
| Creating a Method
168
| NGC Chromatography Systems and ChromLab Software
5.
Depending on the method, either Single Wave UV Detector Settings or
Multi-Wave UV/Vis Detector Settings can be selected. Choose the appropriate
UV absorbance wavelength values to monitor the run.
Tip:
The single-wavelength UV detector monitors UV absorbance one
wavelength at a time. You can select either 255 nm or 280 nm as the
default. The multi-wavelength UV/Vis detector monitors up to four
wavelengths in the UV/Vis range of 190–800 nm.
6.
Under Unit Selection, select the Method Base Unit: volume (ml), time (min), or
column volume (CV).
7.
Under Fraction Collection, click Configure to select a rack from images in the
Configure Fraction Collector Scheme dialog box and choose an available
collection pattern and fraction size.