Priming the ngc system – Bio-Rad ChromLab™ Software User Manual
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| Multicolumn Purifications
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| NGC Chromatography Systems and ChromLab Software
Priming the NGC System
Important:
Whenever you add or remove a module or change or upgrade your
NGC system hardware configuration, you must replumb and reprime the
system.
To prime the system
1.
Power on the NGC system and its connected computer.
2.
To enter manual mode, do one of the following:
On the computer, click Manual Run in the ChromLab Home window or
click the System Control tab and then click in the fluidic scheme.
On the touch screen, click Enter Manual Mode if the application is not
already in manual mode.
3.
(Optional) If your hardware configuration has changed, select a fluidic scheme
that matches your current system configuration.
a.
Select Tools > Change Fluidic Scheme.
b.
Select the appropriate fluidic scheme in the Fluidic Scheme Selector pane.
c.
Click Select to choose the manual mode fluidic scheme.
4.
Insert appropriate inlet lines into the buffer, storage solution, and/or cleaning
solutions to be primed and flushed through the system.
5.
Prime the system.
See Chapter 5, Plumbing and Priming the NGC Chromatography Systems, in
the NGC Chromatography Systems and ChromLab Software Installation Guide
for detailed instructions.